de novo peptide sequencing

De novo peptide sequencing refers to the process of determining the amino acid sequence of a peptide without any prior knowledge of its corresponding DNA or protein sequence. This technique analyzes the mass differences of peptide fragments through mass spectrometry and infers the peptide's amino acid sequence using machine learning models. It is particularly important in proteomics, applicable for studying proteins from organisms with unsequenced genomes, post-translational modifications, and the discovery of new proteins or variants.